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Agar Batata Dextrose Granulado - 500 Gr - Merck

Código: 14541

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POTATO DEXTROSE AGAR GRANULADO SEG HARM EP/USP/JP MERCK
RENDIMENTO 39,0g/L
EMBALAGEM 500G
This culture medium complies with the recommendations of the American Public Health Association for food (1992) and the USP (2003).

Mode of Action
Carbohydrate and potato infusion (BEEVER and BOLLARD 1970) promote the growth of yeasts and moulds while the low pH value partially inhibits the growth of the accompanying bacterial flora. If the medium is to be used for fungal counts, the pH should be adjusted to approximately 3.5. Fungi grow on this medium to
develop typical morphology.

Typical Composition (g/litre)
Potato infusion 4.0 (infusion from 200 g potatoes);
D(+)glucose 20.0;
agar-agar 15.0

Preparation
Suspend 39 g/litre, autoclave (15 min at 121 °C). pH: 5.6 ± 0.2 at 25 °C. If the pH has to be adjusted to 3.5, add approx. 14 ml of a sterile 10 % tartaric acid solution/litre at a temperature of 45-50 °C.
The plates are clear and yellowish-brown.
After the tataric acid is added, do not reliquefy.

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