VRB AGAR (VIOLET RED BILE AGAR) GRANULADO
RENDIMENTO 39,5g/L
Embalagem 500g.

Mode of Action
Crystal violet and bile salts inhibit growth primarily of the Grampositive accompanying bacterial flora. Degradation of lactose to acid is indicated by the pH indicator neutral red, which changes its colour to red, and by precipitation of bile acids.

Typical Composition (g/litre)
Peptone from meat 7.0;
yeast extract 3.0;
sodium chloride 5.0;
lactose 10.0;
neutral red 0.03;
bile salt mixture 1.5;
crystal voilet 0.002;
agar-agar 13.0.

Preparation
Suspend 39.5 g in 1 litre of demin. water and heat to boiling with frequent stirring until completely dissolved. Afterwards do not boil more than 2 minutes.
Do not autoclave. Do not overheat!
pH: 7.4 ± 0.2 at 25 °C.
The prepared medium is clear and dark-red.