Agar Cereus Seletivo Base Mossel (MYP Agar) - 500 Gr - Merck
Código: 14722
CEREUS SELECTIVE AGAR BASE MOSSEL (MYP AGAR) MERCK
RENDIMENTO 21,5g / 450ml H2O
Embalagem 500g.
MYP AGAR (mannitol egg yolk polymyxine agar).
Typical Composition (g/litre)
Peptone from casein 10.0;
meat extract 1.0;
D(-)mannitol 10.0;
sodium chloride 10.0;
phenol red 0.025;
Agar-agar 12.0
Also to be added (per litre of medium):
egg-yolk emulsion 100 ml;
polymyxin B sulfate 100,000 IU = Bacillus cereus Selective Supplement.
NECESSÁRIO SUPLEMENTO 1098750001 E EMULSÃO GEMA DE OVO.
Preparation
Suspend 21.5 g in 450 ml demin. water, autoclave (15 min at 121°C). Cool to about 45 to 50 °C, add 50 ml (this volume can be varied depending on the degree of turbidity desired) of sterile egg-yolk emulsion and the contents of 1 vial Bacillus cereus Selective Supplement, mix. Pour plates.
pH: 7.2 ± 0.2 at 25 °C.
The plates (incl. egg-yolk) are evenly turbid and slightly orange
(red without egg-yolk).