α-Glucosidase from Saccharomyces cerevisiae

Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

Sinônimo(s):

α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast

Número CAS:

9001-42-7

Número da licença da enzima:

3.2.1.20 (BRENDA, IUBMB)

Número EC:

232-604-7

Número MDL:

MFCD00081321

NACRES:

NA.54

tipo

Type I

Nível de qualidade

200

forma

lyophilized powder

specific activity

≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

contém

phosphate buffer salts and EDTA as balance

composição

Protein, ≥50%

características do produto alternativo mais ecológico

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

nº de adesão UniProt

P38158
P53341

atividade externa

β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%

categoria alternativa mais ecológica

Enabling

doença(s) relevante(s)

diabetes (management of non-insulin-dependent diabetes mellitus)

temperatura de armazenamento

−20°C

Gene Information

bakers yeast ... MAL12(853209) , MAL32(852602)

Descrição geral

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Aplicação

α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.[1]

For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters

Ações bioquímicas/fisiológicas

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus[2].

Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.

Embalagem

Sold on basis of p-nitrophenyl α-D-glucoside units.

Definição da unidade

One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.

Nota de análise

Protein determined by biuret.

Outras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer