Guanidine Hydrochloride 99% - 25 Gr - Sigma
Código: 17344
Guanidine hydrochloride
organic base and chaeotropic agent, ≥99% (titration)
Sinônimo(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
Fórmula linear:
NH2C(=NH)NH2 · HCl
Número CAS:
Peso molecular:
95.53
Beilstein/REAXYS Number:
3591990
Número EC:
Número MDL:
ID de substância PubChem:
fonte biológica
synthetic (organic)
Nível de qualidade
400
teor
≥99% (titration)
forma
crystalline powder
aplicação(ões)
RNA extraction: suitable
Impurezas
≤0.3% water (Karl Fischer)
cor
white
pH
(25 °C, 4.6 - 6 at 573 g/L)
pf
180-185 °C (lit.)
solubilidade
H2O: 6 M
densidade
1.3 g/cm3 (lit.)
absorção
≤0.10 at 260 nm in H2O at 6 M
SMILES string
Cl[H].NC(N)=N
InChI
1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
InChI key
PJJJBBJSCAKJQF-UHFFFAOYSA-N
Gene Information
human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)
Aplicação
Guanidine hydrochloride (GdnHCl) has been used:
to perform absolute quantitation of amyloid-beta (Aß) isoforms in plasma
in protein carbonylation assay
for the quantification of glycosaminoglycans (GAG)
for denaturation of cow′s milk allergens
as a control in antiviral screening tests with coxsackievirus B3 (CVB3)
in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Embalagem
25, 100, 500 g in poly bottle
2, 10, 25 kg in poly drum
1 kg in poly bottle
G4505-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.
Ações bioquímicas/fisiológicas
Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule. It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity. GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It is involved in denaturing alkaline phosphatase (ALPase) in Haliotis diversicolor. GdnHCl leads to the deactivation of enzymes like papain and aminoacylase. GdnHCl, at higher concentrations, is effective in virus inactivation, such as herpes simplex virus 1 (HSV-1).
Atenção
May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri™ free-flowing product number RDD001.
Nota de preparo
In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.
Informações legais
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC