Guanidine hydrochloride

organic base and chaeotropic agent, ≥99% (titration)

Sinônimo(s):

Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

Fórmula linear:

NH₂C(=NH)NH₂ · HCl

Número CAS:

50-01-1

Peso molecular:

95.53

Beilstein/REAXYS Number:

3591990

Número EC:

200-002-3

Número MDL:

MFCD00013026

ID de substância PubChem:

24895176

fonte biológica

synthetic (organic)

Nível de qualidade

400

teor

≥99% (titration)

forma

crystalline powder

aplicação(ões)

RNA extraction: suitable

Impurezas

≤0.3% water (Karl Fischer)

cor

white

pH

(25 °C, 4.6 - 6 at 573 g/L)

pf

180-185 °C (lit.)

solubilidade

H₂O: 6 M

densidade

1.3 g/cm³ (lit.)

absorção

≤0.10 at 260 nm in H₂O at 6 M

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

Gene Information

human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)

Aplicação

Guanidine hydrochloride (GdnHCl) has been used:

to perform absolute quantitation of amyloid-beta (Aß) isoforms in plasma

in protein carbonylation assay

for the quantification of glycosaminoglycans (GAG)

for denaturation of cow′s milk allergens

as a control in antiviral screening tests with coxsackievirus B3 (CVB3)

in RNA isolation to dissociate nucleoproteins and inhibit RNase

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Embalagem

25, 100, 500 g in poly bottle

2, 10, 25 kg in poly drum

1 kg in poly bottle

G4505-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Ações bioquímicas/fisiológicas

Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule. It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity. GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It is involved in denaturing alkaline phosphatase (ALPase) in Haliotis diversicolor. GdnHCl leads to the deactivation of enzymes like papain and aminoacylase. GdnHCl, at higher concentrations, is effective in virus inactivation, such as herpes simplex virus 1 (HSV-1).

Atenção

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri^™ free-flowing product number RDD001.

Nota de preparo

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Informações legais

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC