Dulbecco′s Modified Eagle′s Medium - high glucose With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sinônimo(s):

DME, DMEM

NACRES:

NA.75

Nível de qualidade

400

sterility

sterile-filtered

forma

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

L-glutamine: no
glucose: high
NaHCO₃: yes
sodium pyruvate: no
phenol red: yes
HEPES: no

enviado em

ambient

temperatura de armazenamento

2-8°C

Descrição geral

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Embalagem

500, 500, 500 mL

1, 1, 10, 20 L

Aplicação

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Reconstituição

Supplement with 0.584 g/L L-glutamine.