Dulbecco′s Modified Eagle′s Medium - high glucose HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

Sinônimo(s):

DME, DMEM

NACRES:

NA.75

Nível de qualidade

500

forma

powder

technique(s)

cell culture | mammalian: suitable

componentes

sodium pyruvate: no
NaHCO₃: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

enviado em

ambient

temperatura de armazenamento

2-8°C

Embalagem

1, 1, 10, 50 L

1 pkg

Aplicação

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quantidade

Formulated to contain 17.3 grams of powder per liter of medium.

Reconstituição

Supplement with 3.7 g/L sodium bicarbonate.