DAPI for nucleic acid staining

Sinônimo(s):

4′,6-Diamidino-2-phenylindole dihydrochloride, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride

Empirical Formula (Hill Notation):

C₁₆H₁₅N₅ · 2HCl

Número CAS:

28718-90-3

Peso molecular:

350.25

Beilstein:

4894417

Número EC:

249-186-7

Número MDL:

MFCD00012681

ID de substância PubChem:

24894305

NACRES:

NA.52

grau

for molecular biology

Nível de qualidade

300

teor

≥98% (HPLC)

forma

powder

technique(s)

transfection: suitable

solubilidade

H₂O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
PBS: insoluble

ε (extinction coefficient)

30 at 263 nm in H₂O at 1 mM

fluorescência

λ_ex 340 nm; λ_em 488 nm (nur DAPI)
λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)

adequação

suitable for fluorescence

temperatura de armazenamento

2-8°C

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

Descrição geral

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

Aplicação

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Suitable for

DNA staining in agarose gels

analysis of changes in DNA during apoptosis

detection of mycoplasma

photofootprinting of DNA

immunofluorescent staining of cells

DAPI has been used:-

in rapid monitoring of microbial contamination[1]

in chromosomal banding technique[2]

in detection of apoptotic cells[3]

in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA[4]

to stain mature pollen grains(0.5 mg/ml)[5]

Embalagem

1, 5, 10, 50 mg in glass bottle

Ações bioquímicas/fisiológicas

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Atenção

Protect from light.