B-Galactosidase From Aspergillus Oryzae 8.0 Units/Mg Solid - 500.000 Units - Sigma
Código: 19548
B-Galactosidase From Aspergillus Oryzae 8.0 Units/Mg Solid - 25.000 Units - Sigma
Sinônimo(s):
β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae
Número CAS:
Número da licença da enzima:
Número EC:
Número MDL:
forma
solid
Nível de qualidade
specific activity
≥8.0 units/mg solid
contém
dextrin as extender
temperatura de armazenamento
−20°C
Descrição geral
β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.[1]
Aplicação
β-Galactosidase from Aspergillus oryzae has been used:
in in vitro nuclear magnet resonance (NMR)[2]
as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase[3]
to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties[4]
for enzymatic hydrolysis of lactose[5]
for reversed-phase (RP) adsorption[6]
in the hydrolysis of whey lactose[7]
Ações bioquímicas/fisiológicas
β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.[1]
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.[8]
Definição da unidade
One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.
