Oil Red O certified by the Biological Stain Commission

Sinônimo(s):

1-([4-(Xylylazo)xylyl]azo)-2-naphthol, 1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol, Solvent Red 27

Empirical Formula (Hill Notation):

C₂₆H₂₄N₄O

Número CAS:

1320-06-5

Peso molecular:

408.49

Número do índice de cores:

26125

Número EC:

215-295-3

Número MDL:

MFCD00003898

ID de substância PubChem:

24897931

NACRES:

NA.47

grau

certified by the Biological Stain Commission

Nível de qualidade

200

forma

powder

composição

Dye content, ≥75%

pf

120 °C (dec.) (lit.)

solubilidade

chloroform/ethanol (1:1): 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

SMILES string

Cc1ccc(C)c(c1)\N=N\c2cc(C)c(cc2C)\N=N/c3c(O)ccc4ccccc34

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,31H,1-4H3

InChI key

NPGIHFRTRXVWOY-UHFFFAOYSA-N

Descrição geral

Oil Red O is a lysochrome.[1] It is used to stain and visualise general localization of fats.[2]

Oil Red O stains lipid materials and the lipids take red-orange color. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, since alcohol fixation removes lipid.[3][4]

Aplicação

Oil Red O has been used for the staining of lipid droplets and lipid-containing vacuoles in cells.[5][6]

Oil Red O has been used:

for staining liver sections in histological analysis[7][8][9]

for the quantification of lipids in cells[10][11][12]

to stain aorta to examine atherosclerotic lesions[13]

Adequação

Certified for use as a fat stain in Churukian′s modification of the method of Lillie and Asburn.