Deoxyribonucleic Acid From Calf Thymus - 1 Mg - Sigma
Código: 20784
Deoxyribonucleic acid from calf thymus Type XV, Activated, lyophilized powder
Sinônimo(s):
Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
Número CAS:
Número EC:
Número MDL:
eCl@ss:
32160414
NACRES:
NA.52
grau
for molecular biology
Nível de qualidade
tipo
Type XV
forma
lyophilized powder
temperatura de armazenamento
−20°C
Descrição geral
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Aplicação
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels[1] and for DNA quantification by fluorescent assay.[2]
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Características e benefícios
-
No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
Quantidade
One mg DNA is approx. 20 A260 units.
Nota de preparo
Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.[3]
Reconstituição
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.