Deoxyribonucleic acid from calf thymus Type XV, Activated, lyophilized powder

Sinônimo(s):

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

Número CAS:

91080-16-9

Número EC:

293-507-3

Número MDL:

MFCD00151698

eCl@ss:

32160414

NACRES:

NA.52

grau

for molecular biology

Nível de qualidade

200

tipo

Type XV

forma

lyophilized powder

temperatura de armazenamento

−20°C

Descrição geral

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Aplicação

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels[1] and for DNA quantification by fluorescent assay.[2]

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Características e benefícios

• No nuclease activity detected after 16 h at 37 °C incubation

  • High quality template DNA

Quantidade

One mg DNA is approx. 20 A₂₆₀ units.

Nota de preparo

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.[3]

Reconstituição

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.