Bromophenol Blue Sodium Salt - 10 Gr - Sigma
Código: 20975
Bromophenol Blue Sodium Salt
Sinônimo(s):
3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
Empirical Formula (Hill Notation):
C19H9Br4NaO5S
Número CAS:
Peso molecular:
691.94
Beilstein:
6995701
Número CE:
Número MDL:
ID de substância PubChem:
NACRES:
NA.52
forma
powder
technique(s)
electrophoresis: suitable
solubilidade
water: soluble
adequação
suitable for nucleic acid electrophoresis
temperatura de armazenamento
room temp
SMILES string
[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
InChI
1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
InChI key
TWKDJMRHSLWRFI-UHFFFAOYSA-M
Descrição geral
Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Aplicação
Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]
A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.
Reconstituição
A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.