Bromophenol Blue Sodium Salt

Sinônimo(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt

Empirical Formula (Hill Notation):

C₁₉H₉Br₄NaO₅S

Número CAS:

34725-61-6

Peso molecular:

691.94

Beilstein:

6995701

Número CE:

252-170-2

Número MDL:

MFCD00013793

ID de substância PubChem:

57653941

NACRES:

NA.52

forma

powder

technique(s)

electrophoresis: suitable

solubilidade

water: soluble

adequação

suitable for nucleic acid electrophoresis

temperatura de armazenamento

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

Descrição geral

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Aplicação

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis.[1] It has also been used in Drosphila fly food to aid visualization of gut for dissection.[2]

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Reconstituição

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.