Amyloglucosidase

from Aspergillus niger

Sinônimo(s):

Glucoamylase, disaccharidase-type-α-D-glucosidase

Número da licença da enzima:

3.2.1.3 (BRENDA, IUBMB

fonte biológica

Aspergillus niger

Nível de qualidade

100

forma

suspension

specific activity

~14 units/mg protein (At 25 °C with glycogen as the substrate; standardized with BSA.)

peso molecular

M_r 97 kDa

embalagem

pkg of 10 mL (10102857001 [100 mg])

manufacturer/tradename

Roche

Parâmetros

55 °C optimum reaction temp.

pH ideal

4.6-4.8

temperatura de armazenamento

2-8°C

Descrição geral

Amyloglucosidase is synthesized by several Aspergillus genus species.[1] It is a disaccharidase–type α-glucosidase.[2] This enzyme is an exo-enzyme and one of the major industrial enzymes.[3] The stability of amyloglucosidase can be increased by immobilization.[3]

Especificidade

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.
Heat inactivation: Heat inactivation is recommended at 80 °C for 45 minutes, followed by rapidly cooling down.

Aplicação

Amyloglucosidase from Aspergillus niger can be used for the hydrolyzation of terminal α1,4- and α1,6-glucosidic bonds (glucose-glucose bonds) in polysaccharides (e.g., starch, dextrins, glycogen), removing glucose units sequentially from the non-reducing end of the molecule. The enzyme will also cleave maltose and maltosides (maltotriose, maltotetraose, etc.).[4]

Ações bioquímicas/fisiológicas

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides.[5] Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.[6][6]

Definição da unidade

Unit Conversion: One unit (+25 °C; glycogen as substrate) corresponds to 8.6 U (+60 °C; starch as substrate).

forma física

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Outras notas

For life science research only. Not for use in diagnostic procedures.