β-Glycerophosphate disodium salt hydrate - BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)

Sinônimo(s):

BGP, Glycerol 2-phosphate disodium salt hydrate

Fórmula linear:

(HOCH₂)₂CHOP(O)(ONa)₂ · xH₂O

Número CAS:

154804-51-0

Peso molecular:

216.04 (anhydrous basis)

Número EC:

212-464-3

Número MDL:

MFCD00149082

ID de substância PubChem:

329799988

NACRES:

NA.25

linha de produto

BioUltra

Nível de qualidade

400

teor

≥99% (titration)

technique(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

Impurezas

≤0.1% Insoluble matter
≤0.5 mol % L-α-isomer

pf

102-104 °C (lit.)

traços de ânion

chloride (Cl^-): ≤0.05%
sulfate (SO₄^2-): ≤0.05%

traços de cátion

Al: ≤0.001%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.001%
K: ≤0.005%
Mg: ≤0.0005%
Pb: ≤0.001%
Zn: ≤0.0005%

SMILES string

O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O

InChI

1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2

InChI key

ROPZSVKNEIIIDE-UHFFFAOYSA-L

Aplicação

β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.

Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.

Embalagem

10, 50, 100, 500 g in poly bottle

Qualidade

Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.