B-Glicerofosfato (B-Glycerophosphate Disodium Salt Hydrate) 99% - 100 Gr - Sigma
Código: 21139
β-Glycerophosphate disodium salt hydrate - BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)
Sinônimo(s):
BGP, Glycerol 2-phosphate disodium salt hydrate
Fórmula linear:
(HOCH2)2CHOP(O)(ONa)2 · xH2O
Número CAS:
Peso molecular:
216.04 (anhydrous basis)
Número EC:
Número MDL:
ID de substância PubChem:
NACRES:
NA.25
linha de produto
BioUltra
Nível de qualidade
400
teor
≥99% (titration)
technique(s)
cell culture | mammalian: suitable
cell culture | plant: suitable
Impurezas
≤0.1% Insoluble matter
≤0.5 mol % L-α-isomer
pf
102-104 °C (lit.)
traços de ânion
chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%
traços de cátion
Al: ≤0.001%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.001%
K: ≤0.005%
Mg: ≤0.0005%
Pb: ≤0.001%
Zn: ≤0.0005%
SMILES string
O.[Na+].[Na+].OCC(CO)OP([O-])([O-])=O
InChI
1S/C3H9O6P.2Na.H2O/c4-1-3(2-5)9-10(6,7)8;;;/h3-5H,1-2H2,(H2,6,7,8);;;1H2/q;2*+1;/p-2
InChI key
ROPZSVKNEIIIDE-UHFFFAOYSA-L
Aplicação
β-Glycerophosphate is a classical serine-threonine phosphatase inhibitor used in kinase reaction buffers. BGP is often used in combination with other phosphatase/protease inhibitors for broad spectrum inhibition. It functions as an organic phosphate donor and has been used in culture media for mesenchymal stem cell differentiation to osteoblast-type cells. BGP is also used to buffer M17 media for Lactococcus culture in recombinant protein expression.
Initiates differentiation of bone-marrow-derived stem cells embedded in a natural collagen/chitosan gel designed for tissue engineering.
Embalagem
10, 50, 100, 500 g in poly bottle
Qualidade
Low inorganic phosphate content. Mixtures of α- and β-glycerophosphate have historically been used in microbiological media but high levels of inorganic (free) phosphate has been associated with the irregular growth of bacteria and unwanted precipitates.