Spectinomycin Dihydrochloride Pentahydrate - 5 Gr - Sigma
Código: 21728
Spectinomycin dihydrochloride pentahydrate powder, BioReagent, suitable for cell culture
Sinônimo(s):
Spectinomycin
Fórmula empírica (Notação de Hill):
C14H24N2O7 · 2HCl · 5H2O
Número CAS:
Peso molecular:
495.35
Número CE:
Número MDL:
Código UNSPSC:
12352207
ID de substância PubChem:
NACRES:
NA.76
fonte biológica
synthetic (inorganic)
Nível de qualidade
linha de produto
BioReagent
forma
powder
potência
≥603 μg per mg
condição de armazenamento
(Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
técnica(s)
cell culture | mammalian: suitable
cell culture | plant: suitable
Impurezas
endotoxin, tested
cor
white to pale buff
solubilidade
H2O: soluble 50 mg/mL at
espectro de atividade do antibiótico
Gram-negative bacteria
Gram-positive bacteria
aplicação(ões)
agriculture
Modo de ação
protein synthesis | interferes
temperatura de armazenamento
2-8°C
cadeia de caracteres SMILES
O.O.O.O.O.Cl.Cl.CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O
InChI
1S/C14H24N2O7.2ClH.5H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;;;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H;5*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;;;/m1......./s1
chave InChI
DCHJOVNPPSBWHK-UXXUFHFZSA-N
Descrição geral
Chemical structure: aminoglycoside
Spectinomycin has generally been used for the treatment of gonorrhea in males, and a study conducted shows that spectinomycin dihydrochloride pentahydrate provides effective treatment against gonorrhea as well.[1] Clinically, spectinomycin dihydrochloride pentahydrate is easier to use in injections as it shows higher solubility than spectinomycin.[2]
Aplicação
Spectinomycin dihydrochloride pentahydrate has been used as a supplement in different types of media.[3][4]
Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.
Ações bioquímicas/fisiológicas
Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.
Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
Atenção
Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.
Nota de preparo
Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.
Outras notas
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
