Spectinomycin dihydrochloride pentahydrate powder, BioReagent, suitable for cell culture

Sinônimo(s):

Spectinomycin

Fórmula empírica (Notação de Hill):

C₁₄H₂₄N₂O₇ · 2HCl · 5H₂O

Número CAS:

22189-32-8

Peso molecular:

495.35

Número CE:

244-554-3

Número MDL:

MFCD00150886

Código UNSPSC:

12352207

ID de substância PubChem:

24899581

NACRES:

NA.76

fonte biológica

synthetic (inorganic)

Nível de qualidade

200

linha de produto

BioReagent

forma

powder

potência

≥603 μg per mg

condição de armazenamento

(Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)

técnica(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

Impurezas

endotoxin, tested

cor

white to pale buff

solubilidade

H₂O: soluble 50 mg/mL at 

espectro de atividade do antibiótico

Gram-negative bacteria
Gram-positive bacteria

aplicação(ões)

agriculture

Modo de ação

protein synthesis | interferes

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

O.O.O.O.O.Cl.Cl.CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O

InChI

1S/C14H24N2O7.2ClH.5H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;;;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H;5*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;;;/m1......./s1

chave InChI

DCHJOVNPPSBWHK-UXXUFHFZSA-N

Descrição geral

Chemical structure: aminoglycoside

Spectinomycin has generally been used for the treatment of gonorrhea in males, and a study conducted shows that spectinomycin dihydrochloride pentahydrate provides effective treatment against gonorrhea as well.[1] Clinically, spectinomycin dihydrochloride pentahydrate is easier to use in injections as it shows higher solubility than spectinomycin.[2]

Aplicação

Spectinomycin dihydrochloride pentahydrate has been used as a supplement in different types of media.[3][4]

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Ações bioquímicas/fisiológicas

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Atenção

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Nota de preparo

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Outras notas

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.