3,3′-Diaminobenzidine tetrahydrochloride hydrate ≥96%

Sinônimo(s):

(1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate

Fórmula linear:

C₁₂H₁₄N₄ · 4HCl · xH₂O

Número CAS:

868272-85-9

Número CE:

231-018-9

Número MDL:

MFCD08273058

Código UNSPSC:

12171500

ID de substância PubChem:

57654109

NACRES:

NA.47

Nível de qualidade

200

Ensaio

≥96%

forma

powder, crystals or chunks

técnica(s)

titration: 96-104% (with NaOH)

cor

white to light brown, and Faint Red and Faint Grey to Light Grey

pf

280 °C

solubilidade

H₂O: 50 mg/mL

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

chave InChI

DXWSCXIZIHILNP-UHFFFAOYSA-N

Descrição geral

3,3′-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualize the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.

Aplicação

3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).

Ações bioquímicas/fisiológicas

In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.