3,3'-Diaminobenzidine Tetrahydrochloride Hydrate 96% - 50 Gr - Sigma
Código: 21839
3,3′-Diaminobenzidine tetrahydrochloride hydrate ≥96%
Sinônimo(s):
(1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate
Fórmula linear:
C12H14N4 · 4HCl · xH2O
Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47
Nível de qualidade
Ensaio
≥96%
forma
powder, crystals or chunks
técnica(s)
titration: 96-104% (with NaOH)
cor
white to light brown, and Faint Red and Faint Grey to Light Grey
pf
280 °C
solubilidade
H2O: 50 mg/mL
aplicação(ões)
diagnostic assay manufacturing
hematology
histology
temperatura de armazenamento
−20°C
cadeia de caracteres SMILES
O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2
chave InChI
DXWSCXIZIHILNP-UHFFFAOYSA-N
Descrição geral
3,3′-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualize the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.
Aplicação
3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).
Ações bioquímicas/fisiológicas
In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.