Catalase from bovine liver powder, suitable for cell culture, 2,000-5,000 units/mg protein

Sinônimo(s):

H₂O₂:H₂O₂ oxidoreductase

Número CAS:

9001-05-2

Número da licença da enzima:

1.11.1.6 (BRENDA, IUBMB)

Número CE:

232-577-1

Número MDL:

MFCD00081483

Código UNSPSC:

12352204

eCl@ss:

32160410

NACRES:

NA.75

fonte biológica

bovine liver

Nível de qualidade

200

linha de produto

BioReagent

forma

powder

atividade específica

2,000-5,000 units/mg protein

peso molecular

tetramer ~250 kDa

técnica(s)

cell culture | mammalian: suitable

ponto isoelétrico

5.4

nº de adesão UniProt

P00432

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

chave InChI

NUVBSKCKDOMJSU-UHFFFAOYSA-N

Informações sobre genes

cow ... CAT(280743)

Descrição geral

CAT (catalase) is an endogenous antioxidant enzyme, thus conferring protection to cells against damage by ROS (reactive oxygen species). In humans, this gene is localized to chromosome 11p13, which is composed of 12 introns and 13 exons.[1]

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Aplicação

Catalase from bovine liver is used for the following applications:

• Gel filtration[2]

• Analytical filtration on Ultrogel and glycerol gradient centrifugation[3]

• Size exclusion chromatography (HPLC)-HPLC analysis of estrogen receptor[4]

• Enzyme assays[5][6]

• Alcohol oxidase activity[7]

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

• to prepare H₂O₂-O₂ based biocathode for applications in glucose biofuel cells

• to study the kinetic properties and storage stability of catalase immobilized on to florisil

• in glutathione-mediated superoxide generation in an aqueous solution

Ações bioquímicas/fisiológicas

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

This product doesn not need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Atenção

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Definição da unidade

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Nota de preparo

This product is a powder suitable for cell culture with activity at 2,000-5,000 units/mg. The enzyme is soluble in 50 mM potassium phosphate buffer at 1 mg/mL and pH 7.0.