o-Phenylenediamine Peroxidase substrate, ≥98.0%, powder

Sinônimo(s):

1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

Fórmula empírica (Notação de Hill):

C₆H₈N₂

Número CAS:

95-54-5

Peso molecular:

108.14

Beilstein:

606074

Número CE:

202-430-6

Número MDL:

MFCD00007721

Código UNSPSC:

12352204

ID de substância PubChem:

24899004

NACRES:

NA.83

densidade de vapor

3.7 (vs air)

Nível de qualidade

200

pressão de vapor

0.01 mmHg ( 25 °C)

Ensaio

≥98.0%

forma

powder

cor

white to off-white

pb

256-258 °C

pf

98-102 °C

solubilidade

ethanol: 50 mg/mL, clear, colorless to faintly yellow

fluorescência

λ_ex 337 nm; λ_em 417 nm (Pyruvic acid derivative)

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

chave InChI

GEYOCULIXLDCMW-UHFFFAOYSA-N

Descrição geral

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H₂SO₄ and read at 492 nm.

Aplicação

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification.[1] It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.[2][3]

Atenção

May darken in storage.