Ácido etilenodiamino tetra-acético ACS reagent, 99.0-101.0%

Sinônimo(s):

EDTA, EDTA-Na₂, Edatamil, Edetato, Etilenodiamino tetra-acético dissódico, Sequestrene Na₂

About This Item

Fórmula empírica (Notação de Hill):

C₁₀H₁₄N₂Na₂O₈ · 2H₂O

Número CAS:

6381-92-6

Peso molecular:

372.24

Beilstein:

3900609

Número CE:

205-358-3

Código UNSPSC:

12352107

ID de substância PubChem:

24894586

NACRES:

NA.77

grau

ACS reagent

Nível de qualidade

200

Agency

suitable for SM 4500 - NH3

Ensaio

99.0-101.0%

forma

solid

adequação da reação

reagent type: chelator

Impurezas

≤0.005% Insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)

pH

4.0-6.0 (25 °C, 5%)

pf

248 °C (dec.) (lit.)

solubilidade

H₂O: 100 g/L at 20 °C

traços de cátion

Fe: ≤0.01%
heavy metals: ≤0.005% (by ICP-OES)

aplicação(ões)

microbiology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

chave InChI

OVBJJZOQPCKUOR-UHFFFAOYSA-L

Descrição geral

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Aplicação

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

• of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate[1]

• of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)[2]

• of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart[3]

Quelante de cátions divalentes. Inibe enzimas, como as metaloproteases, que requerem cátions divalentes para sua atividade.

Características e benefícios

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Outras notas

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