4-Nitrophenyl N-Acetyl-B-D-Glucosaminide Chromogenic 99% (TLC) - 500 Mg - Sigma
Código: 22094
4-Nitrophenyl N-acetyl-β-D-glucosaminide chromogenic, ≥99% (TLC), powder
Sinônimo(s):
4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside
About This Item
Fórmula empírica (Notação de Hill):
C14H18N2O8
Número CAS:
Peso molecular:
342.30
Beilstein:
96193
Número CE:
Número MDL:
Código UNSPSC:
12352204
ID de substância PubChem:
NACRES:
NA.32
product name
4-Nitrophenyl N-acetyl-β-D-glucosaminide, ≥99% (TLC)
Nível de qualidade
Ensaio
≥99% (TLC)
forma
powder
Impurezas
≤0.05% Free p-nitrophenol
solubilidade
water: 5 mg/mL, clear, colorless to very faintly yellow
temperatura de armazenamento
−20°C
cadeia de caracteres SMILES
CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O
InChI
1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1
chave InChI
OMRLTNCLYHKQCK-DHGKCCLASA-N
Aplicação
N-acetyl-β-D-glucosaminidase (NAG) has been used:
-
as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)[1]
-
as a substrate to study chitinase ChiB1 activity[2]
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to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells[3]
Ações bioquímicas/fisiológicas
4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm.[4] NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.
Substratos
Chromogenic substrate for N-acetyl-glucosaminidase.