4-Nitrophenyl N-acetyl-β-D-glucosaminide chromogenic, ≥99% (TLC), powder

Sinônimo(s):

4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside

About This Item

Fórmula empírica (Notação de Hill):

C₁₄H₁₈N₂O₈

Número CAS:

3459-18-5

Peso molecular:

342.30

Beilstein:

96193

Número CE:

222-398-7

Número MDL:

MFCD00063696

Código UNSPSC:

12352204

ID de substância PubChem:

24897896

NACRES:

NA.32

product name

4-Nitrophenyl N-acetyl-β-D-glucosaminide, ≥99% (TLC)

Nível de qualidade

300

Ensaio

≥99% (TLC)

forma

powder

Impurezas

≤0.05% Free p-nitrophenol

solubilidade

water: 5 mg/mL, clear, colorless to very faintly yellow

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O

InChI

1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1

chave InChI

OMRLTNCLYHKQCK-DHGKCCLASA-N

Aplicação

N-acetyl-β-D-glucosaminidase (NAG) has been used:

• as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)[1]

• as a substrate to study chitinase ChiB1 activity[2]

• to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells[3]

Ações bioquímicas/fisiológicas

4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm.[4] NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.

Substratos

Chromogenic substrate for N-acetyl-glucosaminidase.