Schiff′s fuchsin-sulfite reagent liquid

Sinônimo(s):

Schiff reagent

About This Item

Fórmula empírica (Notação de Hill):

C₂₀H₂₁N₃O₃S

Peso molecular:

383.46

Código UNSPSC:

12171500

NACRES:

NA.47

Nome do produto

Schiff′s fuchsin-sulfite reagent, suitable for detection of glycoproteins

Ensaio

3-5 mM iodine (I) basis (titration)

Nível de qualidade

100

Formulário

liquid

técnica(s)

microbe id | staining: suitable

cor

colorless

solubilidade

water: soluble

densidade

0.990 g/cm³

adequação

suitable for detection of glycoproteins

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

2-8°C

Descrição geral

Schiff’s fuchsin-sulfite reagent is a part of the glycoprotein detection kit. It is primarily used in the Periodic Acid-Schiff (PAS) staining method to detect and visualize aldehydes, glycoproteins in biological samples. The reagent consists of basic fuchsin dye and sodium bisulfite. It reacts with the aldehyde groups of carbohydrates and produces a pink to magenta color, allowing for the identification of these substances in samples.

Aplicação

Schiff′s fuchsin-sulfite reagent has been used:

• in periodic acid-Schiff (PAS) staining to determine goblet cells and salivary glycopreoteins like mucins

• to stain ovaries and testis[1]

• to stain tomato and Medicago primary roots[2]

• to stain undifferentiated sarcomas (USARCs)[3]

It provides a system to easily detect the sugar moieties of glycoproteins on SDS PAGE or Western Blotting membranes.

Nota de preparo

Prepared using pararosaniline.