Ponceau S Powder

Sinônimo(s):

3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt

About This Item

Fórmula empírica (Notação de Hill):

C₂₂H₁₂N₄Na₄O₁₃S₄

Número CAS:

6226-79-5

Peso molecular:

760.57

Número do índice de cores:

27195

Número CE:

228-319-2

Número MDL:

MFCD00003892

Código UNSPSC:

12171500

ID de substância PubChem:

24898418

NACRES:

NA.47

Nome do produto

Ponceau S, BioReagent, suitable for electrophoresis

linha de produto

BioReagent

Nível de qualidade

200

Formulário

powder

técnica(s)

electrophoresis: suitable

cor

red to very dark red

solubilidade

H₂O: 1 mg/mL

ε (coeficiente de extinção)

≥330000 at 518-522 nm in deionized water at 0.015 g/L

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

chave InChI

KQHKSGRIBYJYFX-WNDMICSESA-J

Descrição geral

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.[1]
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Aplicação

Ponceau S has been used for membrane staining (Western blotting.[2][3][4]