Acetaldehyde Assay Kit sufficient for 100 fluorometric tests

Sinônimo(s):

Acetaldehyde Quantitation Kit

About This Item

Código UNSPSC:

12161503

NACRES:

NA.84

uso

sufficient for 100 fluorometric tests

entrada

culture(s)
food(s)
serum
plasma
tissue
urine
beverage(s)

aplicação(ões)

cosmetics
food and beverages

método de detecção

fluorometric

doença(s) relevante(s)

gastrointestinal diseases

temperatura de armazenamento

−20°C

Descrição geral

Acetaldehyde is one of the most widely occurring aldehydes in nature and commonly used in industry. The metabolic byproduct of ethanol in the liver, acetaldehyde is toxic to the human body and rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing known as alcohol flush reaction. Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine.

Aplicação

The Acetaldehyde Assay Kit may be used for:

• Food and Beverage Testing

• Gastrointestinal Disease Research

Características e benefícios

Enhanced Detection Range: Detect Acetaldehyde levels accurately across a wide range, from 0.5 µM to 60 µM, using only 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Adequação

Suitable for biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice)

Princípio

The fluorimetric acetaldehyde assay is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the generated NADH reduces a probe making it fluorescent. The fluorescence intensity of the product measured at λex/em = 530/585 nm is directly proportional to acetaldehyde concentration in the sample.