Biotina 99% (HPLC) Lyophilized Powder

Sinônimo(s):

D-Biotina, Bios II, Coenzima R, Vitamina B7, Vitamina H

About This Item

Fórmula empírica (Notação de Hill):

C₁₀H₁₆N₂O₃S

Número CAS:

58-85-5

Peso molecular:

244.31

Beilstein:

86838

Número CE:

200-399-3

Número MDL:

MFCD00005541

Código UNSPSC:

12352201

eCl@ss:

34058011

ID de substância PubChem:

24891778

NACRES:

NA.21

fonte biológica

synthetic (organic)

Nível de qualidade

300

Ensaio

≥99% (HPLC)

Formulário

lyophilized powder

técnica(s)

HPLC: suitable

cor

white to off-white

pf

231-233 °C (lit.)

solubilidade

ammonium hydroxide: 50 mg/mL (2 M)

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

Descrição geral

Biotin is a water-soluble vitamin. Mammals depend on biotin via plant and microbial dietary sources.[1] Biotin consists of two rings that are attached to valeric acid moiety as a side chain. One of the rings is attached to ureido group and the other ring is the tetrahydrothiophene group.[2]

Aplicação

Biotin may be used to elute proteins from avidin/streptavidin resins. Biotin has been used:

• for culturing of oligodendrocytes.[3][4]
• as a vitamin supplement for the growth of Bacillus species.[5]
• for blocking endogenous biotin during immunohistology procedures.[6][7]

Ações bioquímicas/fisiológicas

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are is required for various applications. Biotin also works as a cofactor/coenzyme of carboxylating enzymes, such as pyruvate carboxylase, acetyl-CoA carboxylases 1 and 2, 3-methylcrotonyl-CoA carboxylase (MCC), and propionyl-CoA carboxylase (PCC).[1] It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A, etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry, and flow cytometry. This approach is also applied for to the purification and characterization of various molecules of interest. Biotin is involved in the epigenetic regulation of genes, cell signaling, and the structure of chromatin.[1]