10x Pcr Buffer Optimized For Routine Pcr With Mgcl2 Included - 1 Vial - Sigma
Código: 23188
Optimized for routine PCR with MgCl2 included
Sinônimo(s):
PCR Buffer Solution, PCR Reaction Buffer
Nível de qualidade
Formulário
liquid
embalagem
vial of 1.5 mL
cor
colorless
aplicação(ões)
agriculture
atividade externa
DNase, RNase, none detected
temperatura de armazenamento
−20°C
Aplicação
10X PCR Buffer has been used as a component of the PCR mixture:
for the amplification of viral DNA[1]
to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals[2]
to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.[3]
Características e benefícios
Standalone buffer
Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)
Nota de preparo
Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin
