Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret

Sinônimo(s):

G-6-P-DH

Número CAS:

9001-40-5

Número CE:

1.1.1.49 (BRENDA, IUBMB)

Número CE:

232-602-6

Número MDL:

MFCD00081656

Código UNSPSC:

12352204

NACRES:

NA.54

fonte biológica

bacterial (Leuconostoc mesenteroides)

Nível de qualidade

200

tipo

Type XXIII

Formulário

ammonium sulfate suspension

atividade específica

550-1,100 units/mg protein (biuret)

peso molecular

54 kDa

concentração

≥2.0 mg/mL Biuret

atividade externa

6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%
PGI ≤0.01%

temperatura de armazenamento

2-8°C

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.[1]Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺).[2] It corresponds to a molecular weight of 54 kDa and exists as a homodimer.[3]

Aplicação

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:

• as a component of reaction mixture for assaying mannose- and glucose-6-phosphate activity[4]

• as a component of reaction mixture in the nevirapine inhibition studies in human hepatic microsomes[5]

• in the glucose-phosphorylating activity of chloroplast extracts[6]

• as a model to test the effect of seed protein fractions on enzyme protection during dehydration.[7]

• in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.[8]

Ações bioquímicas/fisiológicas

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme that protects cells from damage caused by reactive oxygen species by supplying substrates that help prevent oxidative harm.[1] G6PD from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD⁺) or nicotinamide adenine dinucleotide phosphate (NADP⁺) [2] as the first step in the pentose phosphate pathway. Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD⁺.[2] G6PD uses NAD⁺ or NADP⁺ based on the catabolic or anabolic metabolic pathway.[3]G6PD deficiency can lead to acute hemolysis, neonatal jaundice, or severe chronic non-spherocytic hemolytic anemia.

forma física

Suspension in 2.7 M (NH₄)₂SO₄ solution containing 42 mM Tris and 0.8 mM MgCl₂

Outras notas

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.