HIS-Select^® Nickel Affinity Gel (1:1 suspension in a 20% ethanol solution)

Sinônimo(s):

Ni-NTA resin, nickel charged agarose

Sobre este item

Código UNSPSC:

12352200

NACRES:

NA.56

conjugado

magnetic beads

Nível de qualidade

200

Formulário

(1:1 suspension in a 20% ethanol solution)

Características

hydrophilic

embalagem

pkg of 1 mL
pkg of 100 mL
pkg of 25 mL
pkg of 5 mL
pkg of 500 mL

concentração

1.5-2.4 mL/mL (suspension in packed gel)

técnica(s)

protein purification: suitable

cor

faint blue to very dark blue

Matriz

6% Beaded Agarose

capacidade

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

temperatura de transição

flash point 32 °C (closed cup)

temperatura de armazenamento

2-8°C

Descrição geral

HIS-Select^® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.

Aplicação

HIS-Select^® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14),[1] LIM homeobox transcription factor 1 alpha protein,[2] sigma-1 receptor[3] as well as harpin,[4] stable protein 1 (SP1),[5][6] and BCR-ABL fusion protein.[7]

Características e benefícios

High selectivity for higher purity.

Unique non-charged hydrophilic linkage reduces non-specific binding.

Binding capacity for histidine-tagged protein is greater than 15 mg/mL.

Binding under denaturing or non-denaturing conditions.

One-step purification.

Minimizes unwanted ionic interactions.

Minimal nickel leaching.

Bead size: 45-165 μm.

forma física

1:1 suspension in a 20% ethanol solution

Nota de preparo

HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.

Outras notas

It is also available with the EZview™ technology (Product Code E3528).

Informações legais

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany