Atto 565 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sobre este item

Fórmula empírica (Notação de Hill):

C₃₅H₃₄ClN₃O₁₁

Peso molecular:

708.11

Número MDL:

MFCD03456131

Código UNSPSC:

12352108

NACRES:

NA.32

linha de produto

BioReagent

Ensaio

≥90% (HPLC)
≥90% (degree of coupling)

Formulário

powder

fabricante/nome comercial

ATTO-TEC GmbH

adequação

suitable for fluorescence

método de detecção

fluorometric

temperatura de armazenamento

−20°C

Descrição geral

Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.

Características e benefícios

• Strong absorption
• High fluorescence quantum yield
• High thermal and photo-stability

Outras notas

Fluorescence of bioconjugates[1][2]; employed in fluorescence resonance energy transfer, FRET, experiments[3].

Informações legais

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.