Bromocresol Purple

BioReagent, suitable for indicator, Dye content 90 %

Sinônimo(s):

5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form

Sobre este item

Fórmula empírica (Notação de Hill):

C₂₁H₁₆Br₂O₅S

Número CAS:

115-40-2

Peso molecular:

540.22

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24847168

EC Number:

204-087-8

Beilstein/REAXYS Number:

359618

MDL number:

MFCD00011681

Nome do produto

Bromocresol Purple, BioReagent, suitable for indicator, Dye content 90 %

InChI key

ABIUHPWEYMSGSR-UHFFFAOYSA-N

InChI

1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3

SMILES string

Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4

product line

BioReagent

assay

≥89.0% (HPLC)

form

powder

composition

Dye content, 90%

technique(s)

titration: suitable

pH

5.2-6.8, yellow to purple

visual transition interval

5.2-6.8, yellow to blue

mp

240 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL

λ_max

419 nm

ε (extinction coefficient)

≥22000 at 418-424 nm in methanol

suitability

suitable for indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Application

Spectrophotometric determination of serum-albumin

Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.[1]

Biochem/physiol Actions

Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8).[2] It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.[3]