MTT Formazan

MTT reduction end product, powder

Sinônimo(s):

1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan

Sobre este item

Fórmula empírica (Notação de Hill):

C₁₈H₁₇N₅S

Número CAS:

57360-69-7

Peso molecular:

335.43

UNSPSC Code:

12352204

PubChem Substance ID:

329769650

NACRES:

NA.83

MDL number:

MFCD00080731

Nome do produto

MTT Formazan, MTT reduction end product

SMILES string

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

form

powder

solubility

chloroform: 10 mg/mL, clear, dark violet

Quality Level

100

Application

MTT Formazan has been used in cell viability assay.[1][2][3]

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells.[4][5] Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.[6]

Other Notes

Reviews: Tetrazolium salts and formazans[7]