TRI Reagent^®

LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

Sinônimo(s):

TRI Reagent^® Reagente de isolamento de RNA

Sobre este item

UNSPSC Code:

12352200

NACRES:

NA.52

MDL number:

MFCD00213058

Nome do produto

TRI Reagent^®, LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

usage

0.75 mL sufficient for 0.25 mL fluid samples

Quality Level

100

Application

O reagente TRI é uma versão aprimorada do reagente de isolamento de RNA total em uma etapa desenvolvido por Chomczynski. O método de isolamento de RNA baseado neste reagente é amplamente utilizado e aprovado para aplicações com RNA. Ele é ideal para o isolamento rápido, econômico e eficiente de RNA total ou isolamento simultâneo de RNA, DNA e proteínas de amostras de origem humana, vegetal, de levedura, bacteriana e viral.

TRI Reagent^® has been used in RNA extraction.[1][2][3]

The reagent has been used for isolating total RNA.[4] The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.

Features and Benefits

• Isolamento de RNA fácil de escalonar.
• Funciona com várias fontes: humana, vegetal, de leveduras, bacteriana ou viral.
• Melhor rendimento que os métodos tradicionais de tiocianato de gianidina/cloreto de césio.

General description

TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral origin.

A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.

TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.

This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.