Hanks′ Balanced Salts

Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture

Sinônimo(s):

HBSS

Sobre este item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217418

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Balanced salt solution (BSS) provides cells with water, bulk inorganic ions, and buffering system to maintain the normal cell metabolism and physiological range (pH 7.2-7.6), respectively. It also acts as an irrigating, diluting, and transporting agent and maintains the intracellular and extracellular osmotic balance. BSS also provides energy sources such as glucose for cell metabolism.

Application

Hanks′ Balanced Salt has been used:

• to culture primary hippocampal neuron cells from rats[1]
• to collect the rat brains[2]
• to culture rat primary hepatocytes[3]

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.