p-Nitrophenyl phosphate

SIGMAFAST^™, chromogenic, tablet

Sinônimo(s):

pNPP tablets

Sobre este item

UNSPSC Code:

12352204

NACRES:

NA.83

EC Number:

224-246-5

MDL number:

MFCD00284586

Nome do produto

SIGMAFAST^™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL

form

tablet

solubility

water: 0.25 tablet/mL, clear, colorless

storage temp.

−20°C

Quality Level

200

Application

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST^™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Preparation Note

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC