Cytochrome c from bovine heart

≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture

Sinônimo(s):

CYC, CytC

Sobre este item

Número CAS:

9007-43-6

UNSPSC Code:

12352202

EC Number:

232-700-9

NACRES:

NA.61

MDL number:

MFCD00130890

Form:

powder

Assay:

≥95% based on Mol. Wt. 12,327 basis

Biological source:

bovine heart

Mol wt:

12327 Da

biological source

bovine heart

Quality Level

200

assay

≥95% based on Mol. Wt. 12,327 basis

form

powder

mol wt

12327 Da

storage condition

(Tightly closed Dry)

technique(s)

cell culture | mammalian: suitable

impurities

0.40-0.50% Iron (anhydrous)

solubility

water: 10 mg/mL, dark red-brown

UniProt accession no.

P62894

storage temp.

−20°C

Gene Information

cow ... CYC1(512500)

General description

Research area: Apoptosis

Cytochrome c (Cyt C) is an essential mitochondrial protein located in the inner membrane of the mitochondria.[1] It is encoded in the nucleus as apo-cytochrome C.[2] Cyt C occurs as membrane-bound or as soluble metalloproteins in energy-transducing membranes and is found in bacteria, archaea, and plastids.[1] It belongs to the group of class IIa cytochromes and contains pentacoordinate heme centres.[3]

Application

Cytochrome C from bovine heart has been used:

• as a component of the assay solution for testing the activity of respiratory chain complexes III and IV in the mitochondria of human osteosarcoma and human hepatocarcinoma cell lines[4]

• to determine the cytochrome c oxidase activity in mitochondria of human aortic endothelial cells[5]

• as a component of the cyt C oxidase reaction solution during in situ enzyme staining of cyt C oxidase in mice kidney tissues

Biochem/physiol Actions

Cytochrome C (Cyt C) is involved in the activation of caspase during the caspase-dependent apoptosis intrinsic pathway that triggers programmed cell death through apoptosis.[6] It shows lipid-binding activity through its interaction with cardiolipin that accounts for the peroxidase activity of Cyt C.[1] Cyt C binds to heme via the help of cytochrome c heme lyase that enables its release into the mitochondrial intermembrane space.[7]

The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen.

The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.

Preparation Note

Prepared with acetic acid without using TCA.