Meio Murashige And Skoog - 1.000 Ml - Sigma
Código: 6241
Murashige and Skoog Basal Medium powder, plant cell culture tested
form |
powder |
suitability |
plant cell culture tested |
Featured Industry |
Agriculture |
storage temp. |
2-8°C |
Descrição
Formula variant
With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).
Media Formulation
Preparation Note
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium,
such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation.
It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
Quantity
Formulated to contain 4.4 grams of powder per liter of medium.
Application
Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study.[1][2]
General description
Murashige and Skoog medium is a widely used plant tissue culture growth medium.
M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators.
Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).