IMDM - Iscove's Modified Dulbecco's Medium - 500 Ml - Sigma
Código: 21925
IMDM - Iscove's Modified Dulbecco's Medium - With sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture
Sinônimo(s):
Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM
Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.75
product name
Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma
Nível de qualidade
esterilidade
sterile-filtered
forma
liquid
técnica(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
Impurezas
endotoxin, tested
componentes
phenol red: yes
L-glutamine: no
glucose: yes
Condições de expedição
ambient
temperatura de armazenamento
2-8°C
Aplicação
Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Reconstituição
Supplement with 0.584 g/L L-glutamine