IMDM - Iscove's Modified Dulbecco's Medium - With sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

Sinônimo(s):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

Número MDL:

MFCD00217469

Código UNSPSC:

12352207

NACRES:

NA.75

product name

Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

Nível de qualidade

400

esterilidade

sterile-filtered

forma

liquid

técnica(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impurezas

endotoxin, tested

componentes

phenol red: yes
L-glutamine: no
glucose: yes

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Aplicação

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Reconstituição

Supplement with 0.584 g/L L-glutamine